Device
Part:BBa_K1982005:Design
Designed by: Zexu Li Group: iGEM16_NEU-China (2016-09-12)
CRY2-VP64(Prokaryotic LACE system)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 411
Illegal BglII site found at 870
Illegal BamHI site found at 1349 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 295
Illegal AgeI site found at 1024 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 647
Illegal BsaI.rc site found at 56
Illegal SapI.rc site found at 164
Design Notes
no
Source
CRY2 and CIB1 are extracted from Arabidopsis thaliana. tCas9 is from GE Share company. VP64 is synthetic construct.